白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)
Instant ELISA Kit for Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 83-103 | 99 |
| heparin plasma(n=5) | 78-92 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-99% | 80-97% | 83-104% | 92-105% |
| EDTA plasma(n=5) | 94-105% | 81-98% | 84-102% | 85-98% |
| heparin plasma(n=5) | 79-93% | 86-95% | 88-104% | 85-92% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標準品或標本以及同時加入HRP標記白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)的多克隆抗體，孵育結(jié)束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA080Po01 | 白介素8(IL8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA080Po01 | 白介素8(IL8)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA080Po01 | 白介素8(IL8)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA080Po81 | 白介素8(IL8)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA080Po71 | 白介素8(IL8)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA080Po22 | 白介素8(IL8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA080Po24 | 白介素8(IL8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA080Po26 | 白介素8(IL8)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA080Po76 | 白介素8(IL8)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA080Po | 白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA080Po | 白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA080Po | 白介素8(IL8)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA080Po | 白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA080Po01 | 白介素8(IL8)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA080Po01 | 白介素8(IL8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Reproductive Biology and Endocrinology | Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow[Rbej: 14777827] |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa[PubMed: 19958244] |
| Journal of Clinical Periodontology | Experimental peri-implant mucositis at different implant surfaces.[Pubmed: 24521508] |
| Physiological Research | Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.[Pubmed: 2432969] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients[Pubmed: 22461186] |
| J Med Life. | Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination[Pubmed:Pmc4197484] |
| J Clin Periodontol. | Experimental peri‐implant mucositis at different implant surfaces[Pubmed:24521508] |
| British Journal of Nutrition | Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers[Pubmed:24846452] |
| Theriogenology | Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis[Theriojournal:Source] |
| Anim Reprod Sci. | Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes[PubMed: 25851495] |
| Медицина и здравоохранение | ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ[Article: N] |
| BMC Anesthesiology | Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning[PubMed: 25943099] |
| J Breath Res | Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery[PubMed: 25719610] |
| ARTICLE | The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt[Profile: Mohmad_Mosaad] |
| Veterinary Immunology and Immunopathology | Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period[Pubmed:27090626] |
| Infection, Genetics and Evolution | Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression[Pubmed:27349993] |
| Asian Pacific Journal of Tropical Medicine | Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury[science:S1995764516300955] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol[publication:299492265_Diabetes-induced_renal_failu] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells[10610] |
| journal of molecular and cellular cardiology | Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.[pubmed:28088561] |
| Physiological Research | Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury.[pubmed:28006948] |
| PLOS?ONE | Mechanical?Ventilation?Alters?the?Development?of?Staphylococcus?aureus?Pneumonia?in?Rabbit.[pubmed:27391952] |
| The Journal of Comenius University in Bratislava | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury[acm.2017.16.issue-3] |
| Journal of Physiology and Pharmacology | Effects?of?budesonide?on the?lung?functions,?inflammation?and?apoptosis?in a?saline-lavagemodel?of?acute?lung?injury.[pubmed:28195073] |
| 中國藥物警戒 | 小劑量阿奇霉素對穩(wěn)定期老年慢性阻塞性肺疾病患者外周血內(nèi)TNF-α、IL-8、CRP水平及肺功能的影響[Periodical_zgywjj201608004] |
| Veterinary Immunology and Immunopathology | Cytokine levels in colostrum and in foals' serum pre- and post-suckling[pubmed:28242000] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] |
| European Journal of Pharmacology | Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study[pubmed:28684235] |
| PLoS One | Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation[pubmed:29149185] |
| Archives of Oral Biology | The effect of restorative materials on cytokines in gingival crevicular fluid[pubmed:28992599] |
| Experimentelle periimplant?re Mukositis an unterschiedlichen Implantatoberfl?chen[doi: 10.1111/jcpe.12240.] | |
| Molecular?Medicine?Reports | CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB[pubmed:28627614] |
| ARTIFICIAL?CELLS?NANOMEDICINE AND BIOTECHNOLOGY? | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis[pubmed:28608724] |
| Biological Rhythm Research | Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows[10.1080/09291016.2017.1319636] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets[pubmed:28811665] |
| Journal of Ultrasound in Medicine | Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells.[pubmed:28600899] |
| Veterinary Immunology and Immunopathology | Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows.[pubmed:28895861] |
| Biomedicine hub | Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits[10.1159/000475877] |
| Anticancer Research | Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.[pubmed:28982871] |
| Chronic Respiratory Disease | Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort.[pubmed:29117798] |
| Inflammopharmacology | Polar extract of protects cartilage homeostasis: possible mechanism of action[Pubmed:29313174] |
| Life?Sciences | Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusion[Pubmed:29729264] |
| Microbial Pathogenesis | Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos?…[Pubmed:29787791] |
| Lasers in Medical Science | Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears[Pubmed:30003425] |
| Medical Science Monitor | Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)?…[Pubmed:29804125] |
| Journal of Veterinary?Science | Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study[Pubmed:29984902] |
| Bioscience Reports | N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.[Doi: 10.1042/BSR20180858] |
| Acta?Medica?Mediterranea | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung?…[Doi: 10.1515/acm-2016-0012] |
| Journal of?Dairy?Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows[Pubmed: 17430930] |
| Biomedicine?&?Pharmacotherapy | Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits[] |
| Emerging?Microbes?&?Infections | Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin[Pubmed: 30405111] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease[Pubmed: 30573494] |
| International Journal of Chronic Obstructive Pulmonary Disease | Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD[Pubmed: 30655663] |
| Inflammopharmacology | Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &[] |
| Journal of Hematology &?Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment?…[Pubmed: 30621731] |
| BioMed Research International | Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts[] |
| Frontiers in Pharmacology | Naoxintong Capsule Inhibits the Development of Cardiovascular Pathological Changes in Bama Minipig Through Improving Gut Microbiota[Pubmed: 31632272] |
| international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells[Pubmed: 31671764] |
| Scientific Reports | Corrosion Resistance of Graphene oxide/Silver Coatings on Ni–Ti alloy and Expression of IL-6 and IL-8 in Human Oral Fibroblasts[Pubmed: 32094428] |
| Journal of Receptors and Signal Transduction Research | Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathway[Pubmed: 32065019] |
| Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs[Pubmed: 31963653] |
| Physiol Res | Effect of Different Dosages of Dexamethasone Therapy on Lung Function and Inflammation in an Early Phase of Acute Respiratory Distress Syndrome Model[Pubmed: 31928043] |
| Respiratory Research | Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory?…[Pubmed: 31694668] |
| Journal of Pharmacy and Pharmacology | Tectorigenin inhibits inflammation and pulmonary fibrosis in allergic asthma model of ovalbumin‐sensitized guinea pigs[Pubmed: 32314371] |
| Inflammation | Cyanidin-3-O-Glucoside Attenuates Lipopolysaccharide-Induced Inflammation in Human Corneal Epithelial Cells by Inducing Let-7b-5p-Mediated HMGA2/PI3K/Akt?…[Pubmed: 32248330] |
| Sci Rep | NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population[Pubmed: 33177541] |
| International Journal of Nanomedicine | Study on Adenovirus Infection in vitro with Nanoself-Assembling Peptide as Scaffolds for 3D Culture[Pubmed: 32922004] |
| Theriogenology | A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows[Pubmed: 32810791] |
| Cell Cycle | SIRT1 relieves Necrotizing Enterocolitis through inactivation of Hypoxia-inducible factor (HIF)-1a[Pubmed: 32657204] |
| Frontiers in Pharmacology | Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy[Pubmed: 32508661] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?…[] |
| Animals | Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …[] |
| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis[] |
| HMGB1 e citocinas inflamatórias em les?o pulmonar aguda experimental induzida em coelhos[] | |
| Bull Exp Biol Med | Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors[33222085] |
| physiological reports | Impact of synthetic surfactant CHF5633 with SP?\B and SP?\C analogues on lung function and inflammation in rabbit model of acute respiratory distress syndrome[33403805] |
| J Med Food | Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs[33861937] |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population[33850223] |
| Sci Rep | Corrosion Resistance of Graphene oxide/Silver Coatings on Ni¨CTi alloy and Expression of IL-6 and IL-8 in Human O...[32094428] |
| Poultry Science | Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine??-[33518110] |
| Bioactive Materials | Construction of heparin-based hydrogel incorporated with Cu5. 4O ultrasmall nanozymes for wound healing and inflammation inhibition[33778192] |
| animals | Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic??-[34066204] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients[34376749] |
| Pharmaceutics | Nitric-Oxide-Releasing Dexamethasone Derivative NCX-1005 Improves Lung Function and Attenuates Inflammation in Experimental Lavage-Induced ARDS[34959373] |
| Biomedicines | Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes[34829910] |
| Anesthesiology | Adverse Mechanical Ventilation and Pneumococcal Pneumonia Induce Immune and Mitochondrial Dysfunctions Mitigated by Mesenchymal Stem Cells in Rabbits[Pubmed:34965287] |
| J Clin Med | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study[Pubmed:35566605] |
| Microb Pathog | Cell damage and neutrophils promote the infection of Mycoplasma pneumoniae and inflammatory response[Pubmed:35724831] |
| ResearchSquare | LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cells-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axis[] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS[] | |
| Intensive Care Research | Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD[] |
