心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)
High Sensitive ELISA Kit for Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- 編號(hào)HEA478Hu
- 物種Homo sapiens (Human，人)相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍15.6-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于6.3pg/mL.
- 樣本類(lèi)型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格48T96T96T*596T*1096T*100
- 價(jià)格¥ 2587¥ 3696¥ 16632¥ 31416¥ 258720
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-95 | 90 |
| EDTA plasma(n=5) | 90-98 | 95 |
| heparin plasma(n=5) | 79-90 | 84 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 79-90% | 97-104% | 84-95% |
| EDTA plasma(n=5) | 91-99% | 79-89% | 85-103% | 88-102% |
| heparin plasma(n=5) | 82-93% | 86-103% | 84-96% | 90-98% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA478Hu02 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA478Hu61 | 心肌肌鈣蛋白I(cTnI)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA478Hu01 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA478Hu01 | 心肌肌鈣蛋白I(cTnI)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA478Hu61 | 心肌肌鈣蛋白I(cTnI)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA478Hu01 | 心肌肌鈣蛋白I(cTnI)多克隆抗體 | WB; IHC |
| LAA478Hu71 | 心肌肌鈣蛋白I(cTnI)多克隆抗體(生物素標(biāo)記) | WB |
| MAA478Hu28 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| RAA478Hu21 | 心肌肌鈣蛋白I(cTnI)重組抗體 | WB |
| MAA478Hu21 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| MAA478Hu27 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| MAA478Hu23 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB |
| MAA478Hu24 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| MAA478Hu25 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| MAA478Hu29 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu22 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA478Hu26 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB |
| FAA478Hu08 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| FAA478Hu06 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| FAA478Hu04 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| FAA478Hu02 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | Flow cytometry. |
| LAA478Hu74 | 心肌肌鈣蛋白I(cTnI)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA478Hu | 抗心肌肌鈣蛋白I抗體(Anti-cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA478Hu | 心肌肌鈣蛋白I(cTnI)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA478Hu01 | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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