紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- 編號SEA028Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍31.2-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于11.7pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2470¥ 3528¥ 15876¥ 29988¥ 246960
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測紅細胞生成素(EPO)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的紅細胞生成素(EPO)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 86-95 | 91 |
| heparin plasma(n=5) | 78-98 | 86 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的紅細胞生成素(EPO)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中紅細胞生成素(EPO)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-94% | 91-101% | 84-98% | 81-101% |
| EDTA plasma(n=5) | 80-89% | 93-101% | 80-97% | 85-93% |
| heparin plasma(n=5) | 78-103% | 88-95% | 83-91% | 91-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將紅細胞生成素(EPO)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的紅細胞生成素(EPO)與連接于固相載體上的抗體結合，然后加入生物素化的紅細胞生成素(EPO)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的紅細胞生成素(EPO)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA028Hu61 | 紅細胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA028Hu61 | 紅細胞生成素(EPO)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Hu01 | 紅細胞生成素(EPO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA028Hu01 | 紅細胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA028Hu62 | 紅細胞生成素(EPO)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA028Hu06 | 紅細胞生成素(EPO)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA028Hu01 | 紅細胞生成素(EPO)多克隆抗體 | WB; IHC |
| LAA028Hu71 | 紅細胞生成素(EPO)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA028Hu23 | 紅細胞生成素(EPO)單克隆抗體 | WB |
| MAA028Hu26 | 紅細胞生成素(EPO)單克隆抗體 | WB |
| MAA028Hu21 | 紅細胞生成素(EPO)單克隆抗體 | IHC |
| MAA028Hu24 | 紅細胞生成素(EPO)單克隆抗體 | IHC |
| MAA028Hu25 | 紅細胞生成素(EPO)單克隆抗體 | IHC |
| MAA028Hu22 | 紅細胞生成素(EPO)單克隆抗體 | WB,IHC |
| SEA028Hu | 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA028Hu | 紅細胞生成素(EPO)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA028Hu | 紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA028Hu01 | 紅細胞生成素(EPO)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression[PubMed: PMC3509184] |
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| J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness[Pubmed:25324511] |
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| Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin[pubmed:26575329] |
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| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation[pubmed:29147463] |
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| Bulletin?of Experimental?Biology?and?Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia[Pubmed:29308565] |
| Bulletin?of Experimental?Biology?and?Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?…[Pubmed: 30353332] |
| FASEB?JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans[] |
| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats[] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE[] |
| Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind?…[Pubmed: 31743809] |
| BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans[Pubmed: 32866306] |
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| Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness[Pubmed: 32470757] |
| Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia[34367277] |
| J Mater Sci Mater Med | Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules[Pubmed:35072831] |
| Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells[] |
