白介素18(IL18)活性蛋白
Active Interleukin 18 (IL18)
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
- 編號(hào)APA064Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格¥ 1399¥ 3498¥ 6996¥ 20988¥ 52470
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Gene sequencing
SDS-PAGE圖
Figure. Western Blot; Sample: Recombinant IL18, Mouse.
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFγ in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFγ in the cell supernatant was detected using an ELISA kit. INFγ levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.
用法
Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not
vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA064Mu02 | 白介素18(IL18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA064Mu01 | 白介素18(IL18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA064Mu01 | 白介素18(IL18)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA064Mu61 | 白介素18(IL18)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA064Mu01 | 白介素18(IL18)多克隆抗體 | WB |
| PAA064Mu02 | 白介素18(IL18)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA064Mu71 | 白介素18(IL18)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA064Mu21 | 白介素18(IL18)單克隆抗體 | WB |
| MAA064Mu22 | 白介素18(IL18)單克隆抗體 | WB |
| SEA064Mu | 白介素18(IL18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA064Mu | 白介素18(IL18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA064Mu | 白介素18(IL18)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA064Mu01 | 白介素18(IL18)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Cell Death Dis. | MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy[Pubmed:Pmc4237254] |
| PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion[Pubmed:24816434] |
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| PLOS ONE | Protective Effect of Leaf Essential Oil from[PubMed: 25794175] |
| Fertil Steril | Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”[PubMed: 26474737] |
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| Front Pharmacol | Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata[pubmed:27803670] |
| Pharmacological reports | The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder[science:article] |
| International Journal of Molecular?Sciences | PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2[pubmed:27973457] |
| Cellular?Physiology?and?Biochemistry | MiR-155?is?Involved?in?Renal?Ischemia-Reperfusion?Injury?via?Direct?Targeting?of?FoxO3a?andRegulating?Renal?Tubular?Cell?Pyroptosis.[pubmed:28006785] |
| Journal of Food and Drug Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of?Cinnamomum osmophloeum?Kanehira in endotoxin-induced …[S1021-9498(17)30081-9] |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients[pubmed:28655573] |
| Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach[doi:10.4172/2329-888X.1000182] | |
| Cellular Physiology and Biochemistry | Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation[pubmed:28873369] |
| 20 | Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis[pubmed:29024030] |
| Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.[pubmed:28623707] |
| World journal of gastroenterology | Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model.[pubmed:29142469] |
| Veterinary microbiology | Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses.[pubmed:28532803] |
| International immunopharmacology | Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro[pubmed:28605710] |
| Journal of Neuroinflammation | Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage.[pubmed:28732502] |
| Journal of?Neuroinflammation | Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes[Pubmed:29357878] |
| Oxidative?Medicine?and?Cellular?Longevity | The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in Rats[Pubmed:29967666] |
| Journal of Translational Medicine | Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?[Pubmed:29665864] |
| Chemico-Biological?Interactions | Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats[Pubmed:29291386] |
| Journal of?Food?and?Drug?Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice[Pubmed:29389558] |
| International Journal of Molecular?Medicine | MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing?…[Pubmed:29512778] |
| Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China[Pubmed:29305646] |
| Journal of Inflammation | Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus[Pubmed: 30202244] |
| Journal of Affective Disorders | Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study)[Doi: 10.1016/j.jad.2018.10.003] |
| BioScientia Medicina | Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury[] |
| International Journal of Molecular Medicine | Ginsenoside Rg1 protects against H2O2?induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro[Pubmed: 30535505] |
| Nutrients | Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient Rats[Pubmed: 30691017] |
| International immunopharmacology | NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro[Pubmed: 30677569] |
| Acta?Histochemica | Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice[Doi: 10.1016/j.acthis.2019.04.004] |
| JOURNAL OF DRUG TARGETING | Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway[Pubmed: 31094238] |
| IUBMB Life | Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells[Pubmed: 31018046] |
| American Journal of Reproductive Immunology | Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women[Pubmed: 31148259] |
| INFLAMMATION RESEARCH | TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis[Pubmed: 31119302] |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model[Pubmed: 31247004] |
| Journal of Dairy Science | Hepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cows[Pubmed: 31495623] |
| Rheumatology international | Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in?…[Pubmed: 31468124] |
| LIFE SCIENCES | Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathway[Pubmed: 31678278] |
| life sciences | Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome[Pubmed: 31738882] |
| International Immunopharmacology | Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome[Pubmed: 31780368] |
| Journal of Food Science | Effects of Total Flavonoids from Exocarpium Citri Grandis on Air Pollution Particle‐Induced Pulmonary Inflammation and Oxidative Stress in Mice[Pubmed: 31762039] |
| Antioxidants | Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female Rats[Pubmed: 31771099] |
| Frontiers in Immunology | The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the?…[Pubmed: 31921131] |
| Pharmacological Reports | The dual reno-and neuro-protective effects of dimethyl fumarate against uremic encephalopathy in a renal ischemia/reperfusion model[Pubmed: 32141026] |
| Experimental and Therapeutic Medicine | Induction of autophagy protects human dental pulp cells from lipopolysaccharide?induced pyroptotic cell death[Pubmed: 32104285] |
| Cancer Chemother Pharmacol | Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced …[Pubmed: 31989218] |
| Frontiers in Immunology | TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation[Pubmed: 32117301] |
| molecular immunology | STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cells[Pubmed: 32298872] |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors[Pubmed: 32772342] |
| Peptides | Liraglutide ameliorates lipotoxicity-induced inflammation through the mTORC1 signalling pathway[Pubmed: 32771373] |
| molecules | Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages[Pubmed: 32646056] |
| molecular immunology | TLR9 mediates the activation of NLRP3 inflammasome and oxidative stress in murine allergic airway inflammation[Pubmed: 32623292] |
| Inflammopharmacology | Novel complementary coloprotective effects of metformin and MCC950 by modulating HSP90/NLRP3 interaction and inducing autophagy in rats[Pubmed: 32594364] |
| Life Sci | Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome[Pubmed: 32526286] |
| Toxicol Appl Pharmacol | Celastrol augments sensitivity of NLRP3 to CP-456773 by modulating HSP-90 and inducing autophagy in dextran sodium sulphate-induced colitis in rats[Pubmed: 32470352] |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem …[Pubmed: 32406006] |
| Biomedicine & Pharmacotherapy | Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12?…[Pubmed: 32559840] |
| Molecular Medicine Reports | Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats[Pubmed: 32626997] |
| Food and Agricultural Immunology | Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells[] |
| FASEB JOURNAL | Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1‐dependent mitophagy in a Salmonella?…[] |
| Iranian Journal of Allergy, Asthma and Immunology | Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients[] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters[] | |
| Drug Dev Res | Paeoniflorin inhibited nod?\like receptor protein?\3 inflammasome and NF?\|êB?\mediated inflammatory reactions in diabetic foot ulcer by inhibiting the chemokine??-[33236457] |
| Molecules | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion[33430011] |
| Mol Cell Biochem | Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium¨Cglucose cotransporter 1[33608832] |
| Life Sci | Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice[33508297] |
| Tissue Cell | Protective effects of Oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation[33676236] |
| Inflammopharmacology | Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF|êB/AMPK/NLRP3 axis[34002329] |
| Arch Biochem Biophys | Vitamin D ameliorates high-fat-diet-induced hepatic injury via inhibiting pyroptosis and alters gut microbiota in rats[33965368] |
| Placenta | Vitamin D alleviates hypoxia/reoxygenation-induced injury of human trophoblast HTR-8 cells by activating autophagy[34126416] |
| Mol Med Rep | |ê?opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in ?-[34036389] |
| J Mol Cell Cardiol | CircHelz activates NLRP3 inflammasome to promote myocardial injury by sponging miR-133a-3p in mouse ischemic heart[34043986] |
| Med Sci Monit | Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating ?-[34059615] |
| Biological Chemistry | HDAC8 promotes daunorubicin resistance of human acute myeloid leukemia cells via regulation of IL-6 and IL-8[33938176] |
| BioMed Research International | Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves??-[33376718] |
| Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling??-[33505502] |
| BioMed Research International | MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis[33681353] |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity[] |
| Mediators of Inflammation | Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of |á1, 3 ?-[] |
| J Neuroinflammation | Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway[34362408] |
| Mol Med Rep | Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3?induced pyroptosis via the TAK1/JNK signaling pathway[34296299] |
| Int Immunopharmacol | The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages[34332342] |
| LIFE SCIENCES | The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation[34560082] |
| Biochim Biophys Acta Mol Cell Biol Lipids | Elaidic acid induced NLRP3 inflammasome activation via ERS-MAPK signaling pathways in Kupffer cells[34610469] |
| Inflammation | Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo[34787801] |
| J Agric Food Chem | Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats[34694121] |
| Journal of Inflammation Research | The Supportive Role of NSC328382, a P2X7R Antagonist, in Enhancing the Inhibitory Effect of CRID3 on NLRP3 Inflammasome Activation in Rats with Dextran?…[34321905] |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice[34960627] |
| Toxicology | Salidroside alleviates liver inflammation in furan-induced mice by regulating oxidative stress and endoplasmic reticulum stress[34450210] |
| Antioxidants | Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer's Disease[34829535] |
| Ecotoxicol Environ Saf | Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathway[Pubmed:35158278] |
| J Dairy Sci | Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB?…[Pubmed:35248377] |
| Free Radic Biol Med | Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway[Pubmed:35219847] |
| International Heart Journal | Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC Axis[Pubmed:35354753] |
| Journal of Integrative Neuroscience | Gastrodin suppresses pyroptosis and exerts neuroprotective effect in traumatic brain injury model by inhibiting NLRP3 inflammasome signaling pathway[Pubmed:35364660] |
| Perfusion | Insulin reduces pyroptosis-induced inflammation by PDHA1 dephosphorylation-mediated NLRP3 activation during myocardial ischemia-reperfusion injury[Pubmed:35506656] |
| Ecotoxicol Environ Saf | Ambient ozone exposure induces ROS related-mitophagy and pyroptosis via NLRP3 inflammasome activation in rat lung cells[Pubmed:35642860] |
| Medicina | Plasma Concentrations of Cytokines in Patients with Combined Hyperlipidemia and Atherosclerotic Plaque before Treatment Initiation—A Pilot Study[Pubmed:35630041] |
| Pyroptosis Accelerates Spiral Ganglion Neuronal Degeneration Induced by Aminoglycosides in the Cochlea[] | |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling[] |
| Reseach square | Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria[] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS[] |